General Cell Based Assays
Enzo Life Sciences LEADING LIGHT Wnt Reporter Assay Starter Kit (1x96wells)
Chemiluminescent cell-based assay for discovering Wnt pathway modulators. The LEADING LIGHT. .The assay has been used successfully in different assay formats (including HTS applications) to identify several distinct categories of small molecule compounds that modulate the Wnt signaling pathway. Alternative Name: Wnt reporter assay. Applications: FUNC. Chemiluminescence, HTS. Contents: LEADING LIGHT Wnt Reporter Cell Line, Cell Assay and Growth Medium Concentrate, Cell Freezing Medium, Luciferase Substrate and Assay buffer, HEPES, Wnt3a Protein (positive control), and Dkk-1 Protein
Eurofins Discoverx HitHunter cAMP Assay for Small Molecules, 200/800 dp (96-well/384-well). (90-0075SM2)
This detection kit is a robust, sensitive and easy-to-use assay to determine GPCR activity through detection of intracellular cAMP levels. The HitHunter cAMP assay is intended for use with cells expressing Gαs- or Gαi-coupled GPCRs. This product uses EFC technology, and contains ED-tagged cAMP, anti-cAMP antibody, and pure EA. Upon application to a cell which contains endogenous, untagged cAMP, the anti-cAMP antibody will be sequestered by binding to this untagged cAMP, allowing for EFC between the tagged cAMP and soluble EA. This will produce a proportional level of chemiluminescent signal. This kit format is intended for Small Molecules, with a protocol written for use in a 384-well plate. This kit may be used with Eurofins DiscoverX cAMP cell lines as well as cell lines from other sources. This detection kit contains reagents for assaying 2 plates (384-well). For detailed product information, visit www.discoverx.com/products-applications/biochemical-assays.
Eurofins Discoverx PathHunter Flash Detection Kit, 125 (10 plates), 1,000/4,000 dp (96-well/384-well). (93-0247)
The PathHunter Flash Detection Kit is a detection kit intended for use with PathHunter cell lines or with custom cell lines made using ProLabel/PK expression vectors. This kit will provide a brighter signal with shorter kinetics than the PathHunter Detection Kit, at the cost of a shorter half life. It can be substituted in and used with any cell line that specifies use of the PathHunter Detection kit. This kit contains lysis buffer and EFC substrate to allow for detection of EFC levels in the assayed cells. This kit contains all components required to assay 10 plates (96/384-well). For detailed product information, User manual and COA, visit www.discoverx.com/products/reagents/detection-kit-93-0247.
Meso Scale Discovery R-PLEX Human GAS-6/AXL-L ASY
The R-PLEX Human Gas6/Axl-L Assay Kit from Meso Scale Discovery is a multiplex immunoassay for measuring human Gas6 and Axl proteins in serum plasma and cell culture supernatants It uses electrochemiluminescence detection for high sensitivity and broad dynamic range with low sample volume The kit includes a 96-well plate with capture antibodies detection antibodies calibrators and necessary reagents It quantifies Gas6 and Axl crucial for cell survival proliferation migration and phagocytosis in 3 5 hours performing 39 tests in duplicate
Meso Scale Discovery U-PLEX Mouse IL-31 ASY
The Meso Scale Discovery U-PLEX Mouse IL-31 Assay Kit quantifies mouse interleukin 31 (IL-31) in serum plasma and other samples This electrochemiluminescent immunoassay uses MSD s SULFO-TAG labels and multi-array technology for sensitive detection of IL-31 at sub-picogram levels It includes a 96-well plate with capture antibody detection antibody with SULFO-TAG calibrators and diluent The assay runs on MSD s SECTOR Imager providing results in 3 5 hours ensuring reliable quantification of mouse IL-31
TRIBIOSCIENCE INC Glycerol Colorimetric Assay 100 Tests
Tribioscience’s Glycerol Colorimetric Assay kit is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
TRIBIOSCIENCE INC Cytochrome C Oxidase Activity Assay 100 Tests
The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.
TRIBIOSCIENCE INC β-Glucuronidase Assay 100 Tests
Tribioscience’s β-Glucuronidase Activity Colorimetric Assay provides a simple and sensitive method for monitoring glucuronidase activity in biological samples (tissue, cells, serum, urine). This assay uses a synthetic p- nitrophenol derivative (R-pNP) as its substrate. The substrate releases a chromophore pNP under β-Glucuronidase, which can be measured at absorbance (OD 405 nm). The assay can detect as low as 50 µU of glucuronidase activity in a variety of samples.
TRIBIOSCIENCE INC Neuraminidase Activity Fluorometric Assay 100 Tests
The Neuraminidase Activity Fluorometric Assay provides a simple, accurate, and sensitive method for monitoring neuraminidase activity in biological samples. In this assay, neuraminidase cleaves a non-fluorescent substrate to release a fluorescent product, which can be measured at an excitation wavelength of 365 nm and an emission wavelength of 445 nm. Applications - Neuraminidase activity in biological samples. Infuenza virus antigen titration. Key Features - Simplicity - The assay directly detects enzymatic activity without complex enzyme coupling steps. Sensitivity - Even low levels of neuraminidase activity can be detected due to the high fluorescence intensity.
Meso Scale Discovery R-PLEX Human IL-24 ASY
The R-PLEX Human IL-24 Assay QuickPlex 5-PL Kit from Meso Scale Diagnostics is a multiplex immunoassay kit for the quantitative measurement of human Interleukin 24 (IL-24) in serum plasma and cell culture supernatants The kit utilizes electrochemiluminescence (ECL) detection technology and provides reagents for 5-plex analysis of IL-24 in a quick and streamlined workflow The assay has high sensitivity wide dynamic range and requires low sample volume This product can aid in cytokine profiling and research related to cancer and inflammatory conditions
Meso Scale Discovery U-PLEX Human TNF-B ASY
The Meso Scale Discovery U-PLEX Human TNF-beta Assay Kit is a multiplex immunoassay that measures TNF-beta levels in human serum plasma and cell culture supernatants It uses electrochemiluminescence detection technology and provides high sensitivity broad dynamic range and requires low sample volume The assay has high specificity and low cross-reactivity It includes a 96-well plate coated with capture antibodies detection antibodies labeled with electrochemiluminescent tags calibrators and buffer The kit allows simultaneous measurement of TNF-beta and other biomarkers It can be used for biomarker discovery lead optimization and diagnostics